Characterization and Expression of E2 Glycoprotein of Classical Swine Fever Virus in a Eukaryotic Expression System
نویسندگان
چکیده
منابع مشابه
Cloning and Expression of Rabies Virus Glycoprotein Gene into Eukaryotic System
Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...
متن کاملThe E2 glycoprotein of classical swine fever virus is a virulence determinant in swine.
To identify genetic determinants of classical swine fever virus (CSFV) virulence and host range, chimeras of the highly pathogenic Brescia strain and the attenuated vaccine strain CS were constructed and evaluated for viral virulence in swine. Upon initial screening, only chimeras 138.8v and 337.14v, the only chimeras containing the E2 glycoprotein of CS, were attenuated in swine despite exhibi...
متن کاملIdentification of a novel virulence determinant within the E2 structural glycoprotein of classical swine fever virus.
Classical swine fever virus (CSFV) E2 glycoprotein contains a discrete epitope (TAVSPTTLR, residues 829-837 of CSFV polyprotein) recognized by monoclonal antibody (mAb) WH303, used to differentiate CSFV from related ruminant pestiviruses, Bovine Viral Diarrhea Virus (BVDV) and Border Disease Virus (BDV), that infect swine without causing disease. Progressive mutations were introduced into mAb W...
متن کاملcloning and expression of rabies virus glycoprotein gene into eukaryotic system
background and aims: the aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcdna3.1(+) in bsr cell line. this construct might be used for a potential dna vaccine. materials and methods: glycoprotein gene was synthesized and cloned into pbluescript vector and then sub cloned into eukaryotic expression vector (pcdna3.1(+)). after verifica...
متن کاملN-linked glycosylation status of classical swine fever virus strain Brescia E2 glycoprotein influences virulence in swine.
E2 is one of the three envelope glycoproteins of classical swine fever virus (CSFV). Previous studies indicate that E2 is involved in several functions, including virus attachment and entry to target cells, production of antibodies, induction of protective immune response in swine, and virulence. Here, we have investigated the role of E2 glycosylation of the highly virulent CSFV strain Brescia ...
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ژورنال
عنوان ژورنال: Indian Journal of Virology
سال: 2010
ISSN: 0970-2822,0974-0120
DOI: 10.1007/s13337-010-0009-9